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Embryo transfer is gaining popularity in the cattle industry. Great cows are flushed "eggs are removed from her ovary after stimulation" and fertilized, they are then either frozen and can be sold or reimplanted in a donor cow. this allows for individuals to raise/get calves - or puppies - from top-of-the-line breedings and allowing a lesser cow/bitch to raise them.
 

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LH said:
drbobsd said:
Does embryo transfer ever occur in field trial world. Wouldn't one be able to extend a FC female reproductive life or keep her trialing. Maybe its its against the rules just wondering.

Exactly what is embryo transfer? How does it work?
I know the answer to this one - we do this stuff at work. We do it with mice but basically it's the same thing. You take a fertilized embryo(we use 2 cell embryos) and it is surgically implanted in a pseudo-pregnant female mouse and approximately 19 days later you have live born baby mice. We do it with either fresh or thawed frozen embryos.

IVF technician regards,

Andy
 

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It just doesn't work the same in dogs for a variety of reasons.

For example with cattle, they:
- come in heat every 21 days
- normally release 1 egg on ovulation
- gestation length is 9 months & cattle average 1 calf per year.
- can be given homones to induce "super ovulation" or the release of multiple eggs (an average of 8-12) thus in 1 collection will the number of offspring that would normally be produced in her lifetime.
- horomones can be used to time the heat cycle so that multiple recipients can receive the fertilized eggs. (survival rate of frozen embryos is very poor last I knew)
- in theory a cow can be superovulated & collected (8-12 eggs) every 21 day cycle
- I don't know what the record number of calves produced from 1 cow in a year, but I know it is well over 100
 

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Andy Carlson said:
LH said:
drbobsd said:
Does embryo transfer ever occur in field trial world. Wouldn't one be able to extend a FC female reproductive life or keep her trialing. Maybe its its against the rules just wondering.

Exactly what is embryo transfer? How does it work?
I know the answer to this one - we do this stuff at work. We do it with mice but basically it's the same thing. You take a fertilized embryo(we use 2 cell embryos) and it is surgically implanted in a pseudo-pregnant female mouse and approximately 19 days later you have live born baby mice. We do it with either fresh or thawed frozen embryos.

IVF technician regards,

Andy

Sweet, but I DO IT IN HUMANS :lol: Takes a bit longer than 19 days, but in 56% of the cases we get a baby(s) in about 9 months.

Embryologist regards

Andy, who do you get you mouse embryos from? We use them for QC and they aint cheap.
 

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Corey - we make our own. I believe we are the largest supplier of research mice in the world. I work in cryopreservation and we freeze down either embryos or sperm as well as do "recoveries" to produce live born.

Who do you get yours from??

Embryos coming out our ears regards,

Andy
 

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Andy Carlson said:
Corey - we make our own. I believe we are the largest supplier of research mice in the world. I work in cryopreservation and we freeze down either embryos or sperm as well as do "recoveries" to produce live born.

Who do you get yours from??

Embryos coming out our ears regards,

Andy
We get them from Embryotech
 

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embroys

I know Nick Elam, DMV who is reproductive specialist is working very hard on this very subject. He is working with some experts in the cattle industry and some other scientists to reproduce FT dogs. It would help the progression of improving our breed by getting more progeny from proven bitches.
CB
 

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Nick Elam has mentioned going across the Atlantic to research this in the past. Don't know where they are with it, but it is geing worked on.
 

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LH said:
drbobsd said:
Exactly what is embryo transfer? How does it work?
Wish it was done in dogs.
I specialized in ET with cattle 20 yrs. ago.
BASICALLY(VERY)
The cow is given a hormone to make her come in heat(lutylase 20 yrs ago).
She is "drugged" to cause superovulation.
She is a.i.'d..
The uterus is flushed 7 days later.
The quality embryo's are transfered to recipient cows whose heats have been syncronized with the momma. Those cows carry the embryo's to full term. The blood of the embryo is not mixed with the blood of the cow.
20 years ago, most flushings resulted in 2-4 pregnancies. Although we did one in Texas where the cow released 64 eggs, 47 were fertilized, 29 were of good enough quality to be transfered to a recipient cow, and 9 months later 23 calves were on the ground. That mama cow was retired.

I've discussed this with my vet along with the Miss. State Vet School and they say it is being researched, but not "real" close yet.

The college I attended had a Chianina bull embryo shipped over from Europe in a rabbit, then transfered it to a recipient cow here. It ended up about 2800 pounds. The rodeo team had fun trying to ride that big dude. The world record is 3800 pounds.
 

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drbobsd said:
Does embryo transfer ever occur in field trial world. Wouldn't one be able to extend a FC female reproductive life or keep her trialing. Maybe its its against the rules just wondering.
It's done with big-time show horses when you don't want an expensive competition mare down with being preggers for months on end, but haven't heard of it being done with dogs.
 

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Wisconsin Equine Center, here outside of Oconomowoc, does this with Thoroughbred Race Horse Mares. Serigents(sp?) are used and this type of breeding is quite sucessful.

What I have been told by the specialist there; Canine implantation is a way off yet. Nothing more specific, sorry.
 

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The last time I was at Nick's (about four months ago) he had successfully flushed and frozen some dog embryos. He had just thawed them that morning and they were indeed "alive".... I think he is closer than some might think to be able to take a flushed and frozen embryo to full term.

Wonder how long it will take the AKC to come up to speed on registering litters that are conceived this way.

Wendy Bonello
Cresthill Kennels
________
Lincoln mkx picture
 

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Cresthill said:
The last time I was at Nick's (about four months ago) he had successfully flushed and frozen some dog embryos. He had just thawed them that morning and they were indeed "alive".... I think he is closer than some might think to be able to take a flushed and frozen embryo to full term.

Wonder how long it will take the AKC to come up to speed on registering litters that are conceived this way.

Wendy Bonello
Cresthill Kennels
Hope AKC comes around a little faster than the Jockey Club - a shame that Barbaro won't live on because of their quaint, archaic rules.
 

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WOW...a actual excuse to have "brood bitchs" around :?

Keep the MaMa's home...they need time out too...Our sport will Never be the same when/if this ever comes to pass...I dread the day :(
 

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We do it ALL the time with cutting horses. It's still not allowed w/thouroughbreds. It's very useful if you have a mare that has trouble foaling but has nice foals, just get an embryo out of her and place it in a recipient mare and then your problem is solved, or if you have a nice mare you just want to keep showing but get some babies out of her, go embryo. If you want to get multiple babies out of one mare a year, go embryo. It's not uncommon to see 2-3 babies of the same year at the big shows. It also makes it so that mares who are getting old, you can get as many foals out of them as possible. Boon San Sally died two years ago but still had babies the following year. I'm not 100% sure I agree with it, but it's getting to the point where if you don't do it, you aren't going to be able to keep up, I hope Labs never get to be that way. The high selling weanling this year at the NCHA Futurity went for $750,000 and she was an embryo transfer baby.
 

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Targander said:
WOW...a actual excuse to have "brood bitchs" around :?

Keep the MaMa's home...they need time out too...Our sport will Never be the same when/if this ever comes to pass...I dread the day :(
LeAnne, to clarify, my comments re Barbaro were aimed at the JC because they still require thoroughbreds to be bred via 'live cover', ie no AI.

However, I would expect that the Jockey Club wouldn't go for embryo transfer either, so a quick search of the website yields....surprise, surprise....

Breeding Practices Not Approved by The Jockey Club

Artificial Insemination: The process of depositing semen into the reproductive tract of a broodmare in order to get a broodmare in foal (pregnant) without the physical mounting by a stallion.

Cloning: Any method by which the genetic material of an unfertilized egg or an embryo is (i) removed, (ii) replaced by genetic material taken from another organism, (iii) added to with genetic material from another organism, or (iv) otherwise modified by any means in order to produce a live foal.

Embryo Transfer (Transplants): The method whereby a developing embryo or unfertilized egg is removed from its natural dam and implanted into the reproductive tract of either the natural dam or a host dam for a portion of the gestation period in order to produce a live foal.
 
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